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Keep lyophilized vials cold until use, then reconstitute with sterile diluent under aseptic technique immediately before an experiment

Acknowledgements The authors are grateful for the encouragement of Professor Jie Bai to undertake this review

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It enters the airway epithelium, inhibits the host innate interferon (IFN) immune response, and starts replication due to activation of the nuclear factor kappa B (NF-) transcription pathway ( Coronavirus being an RNA virus undergoes continuous mutation leading to alteration in the protein sequence of its spike and within itself