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Data processing and normalization for semi-targeted and targeted metabolomics For targeted metabolomics analysis, relative quantitation of polar metabolites was performed using a combination of TraceFinder version 5.1 (Thermo Fisher Scientific, Waltham, MA) and Compound Discoverer (CD) version 3.3 to increase annotation efficiency and data completeness

The process is identical for both: For Retatrutide (10 mg vials) , add 2 mL of bacteriostatic water to create a concentration of 5 mg per mL

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Covington, H

The timing, frequency, and length of treatment depend on the specific peptide used