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The viability of U87-MG and IR R-1 cells after IR stress and under Rho inhibition is linked to the formation of H2AX foci: IR-resistant clones showed more H2AX foci under stress-free conditions than did U87-MG cells, but this DNA damage sensitivity was rapidly reversed after IR and was still decreased by C3 toxin treatment in parental cells but not in IR R-1 clones (Fig

If using a pen with replaceable needles, attach a new needle according to the manufacturer's instructions, remove both the outer and inner needle caps, and perform a flow check/priming if required by your specific device

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