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The effective blood-brain barrier mechanism impeding the transport of monoamines has been attributed to both the presence of tight junctions cementing adjacent endothelial cells of the cerebral capillary walls, along with high concentrations of dopa decarboxylase and monoamine oxidase (MAO) in the cerebral capillary endothelium (Bertler et al, 1966

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For AtSNX1 primary antibody, AtSNXl cDNA was cloned into the pET28a plasmid, which incorporates an N-terminal histidine (His) tag into the expressed protein