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Patient enrollment began in January 2014 and ended when the target sample size was reached in September 2014

To assess whether macrocycles and hHGF interacted with human, mouse and canine Met, each biotinylated macrocycle or hHGF was immobilized to a surface density of 200500 RUs (for biotinylated macrocycles) or ~2,200 (for biotinylated hHGF) RUs on a CAP sensor chip using a Biotin CAPture Kit (GE Healthcare), following the standard protocol

In addition, riboflavin-dependent enzymes have essential roles in pyridoxine activation, tryptophan-kynurenine pathway, and homocysteine metabolism

Okada F, Shionoya H, Kobayashi M, Kobayashi T, Tazawa H, Onuma K, et al