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Such increased permeability can also stimulate immune cells such as the macrophages and dendritic cells to release inflammatory cytokines, and enhance antigen presentation for possible autoimmune conditions (Ullah et al., 2024)

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The capillary voltage was set in a range of 22 to 25 V, the spray voltage was between 3.00 and 4.50 kV, and a capillary temperature of 180 C was employed