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23 ChenC.-M.WuC.-C.KimY.HsuW.-Y.TsaiY.-C.ChiuS.-L

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In pre-ASD newborns, several sphingolipid metabolic pathways and chenodeoxyglycocholic and taurodeoxycholic bile acids are increased, whereas serotonin, dopamine, niacin, vitamin B3, and flavin adenine dinucleotide are decreased [315]

Briefly, the islets were incubated in RPMI 1640 Medium (Gibco) at 37 C overnight, before they were washed in Krebs-Ringer HEPES (KRH) solution with 2.8 mM glucose (low-glucose buffer)

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