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Lentivirus of the Brunello CRISPR library was produced in HEK293T cells by co-transfection of the sgRNA pooled library in LentiCRISPRv2 plasmid together with pMD2.G and psPAX lentiviral helper plasmids using Xfect as the transfection reagent in T225 flasks 17

Biotin is found in many foods, but the assessment of its dietary intake is hampered by the absence of biotin in nutrient composition databases

Effect of natural and synthetic antioxidants in a mouse model of chronic fatigue syndrome

Cycling matters most for the GH axis

Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans