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3 H-Leucine incorporation assay To estimate the rate of protein synthesis, cells were cultured in DMEM containing L-[4,5- 3 H] leucine (1 Ci/ml, PerkinElmer) which incorporates into newly synthetized proteins

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Allergies Check for hypersensitivity before use Inform your doctor about any previous allergic reactions to B vitamins, cobalt, or injectable medications