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Binding/Wash Buffer: PBS, pH 7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH 7.4) Elution Buffer: 50 mM Tris-HCl, 10-20 mM reduced glutathione, pH8.0 2.2 Preparation of the Magnetic Beads 1) Completely resuspend the beads by shaking or vortexing the vial

However, researchers now understand the condition involves much broader systemic dysfunction

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