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Recombinant mouse HGF (carrier protein-free) was incubated with GSSSG (A) and LASSS (B) at molar ratios ranging from 1:0 to 1:4000 relative to HGF (corresponding to weight ratios of 1:0 to 1:57 and 1:0 to 1:21 with A and B, respectively) just prior to the optimized peroxynitrite-protocol at a molar ratio 1:2000 relative to HGF, pH 7.4 (Figure 1b,c)

doi: 10.3390/medicina57030200 589 NaculLAuthierFJScheibenbogenCLorussoLHellandIBMartinJAet al

Exercise and injection site selection Exercise increases blood flow to working muscles and surrounding tissue

It is also possible that radical intermediates nonenzymatically produced from hydroperoxy-phospholipids directly react with GSH, leading to the formation of a highly pro-oxidant GS radical

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