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Development of Incretinbased Therapies As the insulinotropic effects of GLP1 are (partially) preserved in T2D, most pharmacological efforts have focused on making use of the preserved ability of GLP1 to stimulate the GLP1R (rather than the functionally ineffective GIP receptor) to reintroduce incretin activity 8

Funding : Novo Nordisk, manufacturer of semaglutide, designed and executed the study as well as funding the study

To investigate this possibility, we used HEK293 cells stably expressing a genetically-encoded calcium sensor GCaMP6s, which exhibits increased green fluorescence upon an increase in cytosolic calcium levels 17

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HaCaT keratinocytes have also been proven to be good candidates for assessing skin irritancy and toxicity in conventional monolayer assays measuring using MTT reduction as the viability endpoint 21