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It was recently shown that antioxidants can prevent loss of HSCs self-renewal potential in several contexts, such as aging or after exposure to low doses of irradiation, suggesting that antioxidants can be used to maintain HSCs functional properties upon culture-induced stress [20, 21]

Metal-labelled antibodies, MAXPAR X8 labeling reagent kits and Cell-ID IdU were purchased from Fluidigm Corp (South Francisco, CA, USA)

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The compositions of these media are listed in Supplementary Table 2