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Sensitive and accurate: linear detection range 0.4 - 100 M in 96-well plate Simple and convenient: mix the DTNB reagent with sample, remove protein precipitates for proteinaceous samples, add a second reagent and read the optical density Low interference: amino acids and common buffers do not interfere Direct assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts Drug discovery/pharmacology: effects of drugs on glutathione metabolism Note: The below protocol is a sample protocol

doi: 10.1038/s41598-024-84395-z 56 JiangM.LiR.LyuJ.LiX.WangW.WangZ.et al

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[DOI] [PMC free article] [PubMed] [Google Scholar] 104.Khodaie L., Sadeghpoor O