For the gender comparisons with difference scores, data was analyzed with separate Mann Whitney rank sums tests

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The cells were then washed with PBS, fixed in 4% paraformaldehyde (100 L/well), and nuclei were stained with 40 M of 4, 6-diamidino-2-phenylindole (DAPI)
Insulin secretion in isolated fetal islets was not different based on infusion at the time of islet collection
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