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All experiments with sygl-1(ubiq) and lst-1(ubiq) were carried out at 15C to maximize tumor penetrance, except those in S4A and S4B Fig , where tumor penetrance was tested with different temperature regimens, and in S5ES5J Fig , where epistasis with fbf-1 fbf-2 was assayed at 25C

tirzepatide (a GLP-1/GIP dual agonist) also uses weekly dosing but activates an additional receptor pathway, which some patients tolerate better

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Nat Rev Endocrinol 6:689697 Perea A, Clemente F, Martinell J, Villanueva-Penacarrillo ML, Valverde I (1995) Physiological effect of glucagon in human isolated adipocytes