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Cells were broken by homogenization with 0.45 mm glass beads in the Bead-Beater (Biospect Products Bartelsville, Okla) kept on ice bath for 20 min, with cycles of 1 min treatment

Once confined primarily to academic laboratories, bioactive peptides are now widely examined for their potential roles in tissue regeneration, metabolic regulation, and cellular signaling

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While clear synergy was not demonstrated across all tested isolates, the absence of antagonism supports the rationale for further preclinical investigations, and potentially future clinical studies [93]