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At day 4 post infection, cell supernatant containing the released virus was harvested, centrifuged at 500 g for 5 min, filtered through a 0.22 M sterile vacuum filtration system, aliquoted and stored at 80 C until further use

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The first known use of vapes dates back to 1964 when Herbert Gilbert filed a patent for his smokeless non-tobacco cigarette after witnessing his brother smoking from an electronic pipe that he had invented himself