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For lung tissue lactate measurement, equal amounts of lung tissue were homogenized with sterile cold PBS and centrifuged, and cell-free supernatants were collected

Basic Workflow: -Sample injection into LC system -Separation of components via chromatography -Ionization (typically ESI) -Mass detection (MS) -Optional fragmentation (MS/MS) for structural analysis Used extensively in pharmaceutical, biotech, and proteomics research 4

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