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THP-1 cells were seeded at 2 10 5 cells per well in complete RPMI 1640 medium with 100 nM phorbol 12-myristate 13-acetate (PMA) (Cayman) and differentiated into macrophages for 3 days, then rested in PMA-free medium for 16 h before infection

This is considered more realistic than pure in vitro testing because the natural architecture and cell-to-cell communication is maintained

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Promotes delta-wave deep sleep, regulates circadian rhythms, and enhances stress recovery through sleep architecture optimization and HPA axis modulation

A 2025 prospective study on polynucleotides in androgenetic alopecia reported improvements after four treatment sessions