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Therefore, properly recovering the FAO after insult is crucial, as a defect in renal FAO leads to lipid accumulation and/or reduction of FAO likely participates in the mesenchymal reprogramming of renal epithelial cells, elevating the risk of developing CKD from even transient AKI [139,140,141]

Lowering GSH levels also triggers ferroptosis through deactivation of GPX4, leading to accumulation of lipid peroxides [87] In some cases, GSH depletion alone can induce therapeutic effects without relying on Fenton chemistry, which typically involves HO reacting with Fe 2 to form hydroxyl radicals (OH) that damage critical biomolecules such as DNA, proteins, and lipids

Shaking the vial : Denatures the peptide

Free Radic Biol Med 45(8):11431149

MCA1568GA) and a viability marker (propidium iodide, Sigma-Aldrich, Merck, Cat No