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Measurement of reactive oxygen species (ROS) and reduced glutathione (GSH) After maturation, the oocytes were incubated in 10 M CM-H 2 DCFDA (Thermo Fisher Scientific, Barcelona, Spain) and 10 M Cell Tracker Blue (Thermo Fisher Scientific, Barcelona, Spain) for 30 min at 37 C in the dark to detect intracellular reactive oxygen species (ROS) and reduced glutathione (GSH), respectively

5 mg Dose: Draw 50 units (0.5 mL)

Withdrawal is a neurological event driven by the brains chemical adaptation to alcohol

The results showed cytotoxicity at specific concentrations, with higher doses of SWNTs, CB, and C60 causing cell death, membrane damage, and vacuole formation

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