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Materials and methods Cell culture Human acute monocytic leukemia cells, THP1, and the mouse leukemic monocyte cell line, RAW264.7, were cultured using RPMI-1640 medium modified to exclude calcium nitrate and supplemented with 2.05 mM L-Glutamine (Gibco, C11875500BT), 10% fetal bovine serum (FBS) (ExCell, FSP500), and a 1% penicillinstreptomycin mixed solution (Abiowell, AWH0529a)

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Among the complexes, the MRP7-sertraline complex had the most negative free energy of binding value (43.17 kcal/mol), whereas the MRP1-fluoxetine complex had the least negative value (33.64 kcal/mol)