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Human clinical trial data remains limited, with Phase I and II trials for inflammatory bowel disease conducted in the 1990s claiming safety with no toxicity, though full peer-reviewed data never appeared in scientific literature

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The dose-response curve for neurotrophic factors is not linear, and more is not always better

During the procedure the animal is placed on a heating pad (40 C.) in a sound proof booth and an earphone is fitted loosely into one ear at a time