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In addition, when co-cultured with human breast cancer cells, neutrophils produced high levels of oncostatin M (OSM) (84), which was shown to act on the endothelium via STAT-3/VEGF signaling in vivo and to enable the remodeling of the TME by activating cancer-associated fibroblasts (CAF) (85, 86)

Pushing beyond this timeframe introduces a significant risk of using a less potent, partially degraded compound, which will directly impact the reliability of your experimental data

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