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Pharmacokinetic interactions and first-pass metabolism drugs absorbed from the gut (with the exception of the buccal and rectal mucosa) pass via the portal vein to the liver where they may be subject to first-pass metabolism

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as pigment-producing stem cells are depleted, greying is irreversible

Each liver was homogenized in 3ml of 0.25 M sucrose per g of liver, and centrifuged at 3,000 and 10,000 rpm for 10 minutes respectively to precipitate cell debris