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Cells were harvested using 110 L ice cold lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche)

Each route impacts how quickly the compound is absorbed, distributed, and eliminated

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Supplies you need 30 mg tirzepatide vial (lyophilized powder) Bacteriostatic water (not sterile water, not saline) 3 mL syringe with 18-22 gauge needle for drawing water 1 mL insulin syringes (29-31 gauge) for dosing Alcohol swabs Clean, flat work surface Step 1: Preparation Wash your hands thoroughly