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Typical applications include glutathione S-transferase phase-II conjugation assays, glutathione peroxidase and glutathione reductase coupled assays, GSH/GSSG ratio determination as a redox readout in cultured cells, protein S-glutathionylation studies, and ferroptosis models where glutathione availability governs glutathione peroxidase 4 activity and therefore phospholipid hydroperoxide handling (Yang et al., Cell, 2014)

XL413, an inhibitor of CDC7 kinase, effectively induced senescence in liver cancer cells, simultaneously sensitizing them to the senolytic action of the mTOR inhibitor, AZD8055 [136]

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The resulting cDNA was diluted 100 folds, and 1.2 l primer and 5 l 2X SYBR Green were mixed before loading into a 384-well plate