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After electrophoresis, gels were washed for 10 min with 50 mM phosphate buffer, pH 7.8, and then incubated in a solution containing 50 mM potassium phosphate (pH 7.8) containing 275 g/mL nitro blue tetrazolium (NBT), 65 g/mL riboflavin, and 0.25% tetramethylenediamine (TEMED)

The treatments were performed for 72 h, and for the final 6 h of treatment, H 2 O 2 substrate solution was added to the wells, and 2 h before the end of the treatment, 100 L of ROS-Glo detection solution was added to each well