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The homogenates were centrifuged at 15,000 rpm for 10 min, and the protein concentration in the supernatant was determined using the Pierce Bicinchoninic Acid assay Kit (Thermo Scientific)

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However, an alternative explanation, albeit not mutually exclusive, is direct interaction of GLP-1RAs with GLP-1R in brain regions such as the brainstem (area postrema, nucleus tractus solitarii), which are not protected by the blood-brain barrier, and are typically involved in medication-induced nausea (chemosensitive area) (5456)

The Time-Feature of Uric Acid Excretion in Hyperuricemia Mice Induced by Potassium Oxonate and Adenine

Your body produces it in the kidneys and liver and stores it in the heart, brain, sperm, and skeletal muscles