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However, some patients continue to experience attacks despite prophylactic treatment

Cloning the hGrx1-roGFP2 constructs The hGrx1-roGFP2 coding sequence [69] was amplified using the forward (Grx1 primer: 5 - AGTCGGTACCATGGCTCAAGAGTTTGTGAACT - 3) and reverse (roGFP2 primer: 5- AACCCCCGGGTTACTTGTACAGCTCGTCCATG -3) primers and cloned into the pARL+ expression vector [34], using Kpn I and Xma I restriction sites (underlined)

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