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doi: 10.3389/fphar.2026.1791206 Received 19 January 2026 Revised 13 April 2026 Accepted 14 April 2026 Published 01 May 2026 Volume 17 - 2026 Edited by Kabirullah Lutfy, Western University of Health Sciences, United States Reviewed by Marsida Kallupi, University of California, San Diego, United States Christina Merritt, University of Texas Medical Branch at Galveston, United States Updates Copyright 2026 Hrickova, Demirci, Amchova and Ruda-Kucerova

Step 6: label and store Write the following on the vial or a label: Reconstitution date Concentration (e.g., 3.33 mg/mL) Expiration date (28 days from reconstitution) Place the vial in the refrigerator immediately

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All medications have side effectsMost of these are mild and short-lived, such as nausea or bowel habit changes

Prospective Covariate Control: Designing adequately powered prospective longitudinal studies capable of testing whether baseline microbial signatures predict treatment outcomes while strictly controlling for diet, metformin use, and weight loss dynamics