After 15 min of exendin 2029 or exendin-4 administration, a glucose solution (2 g/kg) was intraperitoneally injected (at 0 min
The plates were then washed twice with ice-cold PBS, scraped, pelleted 250 g at 4 C for 3 min and lysed with RIPA buffer (50 mM Tris-Cl (pH = 8), 150 mM NaCl, 5 mM EDTA, 1 % NP-40, 0.5 % sodium deoxycholate, 0.1% SDS, 1x protease and 1x phosphatase inhibitors)
In addition, the expression levels of menA and ubiE , the genes encoding key enzymes involved in the conversion of chorismate to VK2 in the intestinal microbiota, were significantly increased after the addition of C
Molecules 27, 4058
Additionally, untargeted metabolomics has been applied to assess serum metabolite changes induced by microbial interactions