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After fixing with 2% PFA and 0.5% glutaraldehyde for 10 min at room temperature, GFP+ CSMN were identified under epifluorescent microscope, and their location on the grid marked before processing for EM analysis

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Why GLP-1s are Biologically Plausible for Alzheimers Beyond lowering glucose and weight, GLP-1 signaling appears to: Improve insulin signaling in the brain Dampen neuroinflammation (microglial activation) Reduce oxidative stress Interact with amyloid- and tau pathways in preclinical models Recent peer-reviewed reviews synthesize these mechanisms and frame GLP-1s as potential multi-target neuroprotective agents rather than single-pathway drugs

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