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Key validation metrics include pharmacokinetics/pharmacodynamics concordance (e.g., clearance rates, area under the curve [AUC], metabolite formation), toxicological sensitivity and specificity, and the recapitulation of clinical-grade disease phenotypes and drug responses

Collectively, these untargeted metabolomic analyses indicate subtle but biologically relevant metabolic differences between ASD and control groups, characterized by increased variability and pathway-level alterations rather than complete global separation

Any concerns or questions are addressed before treatment begins

10.1186/2046-4053-3-124 80 TianX.WangQ.GuoR.XuL.ChenQ

Peer review Peer review information Communications Biology thanks Changhong Du, Eun-Woo Lee and the other, anonymous, reviewer(s) for their contribution to the peer review of this work