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Mice were mounted on a stereotaxic apparatus with 2% isoflurane (Panion & BF Biotech), and their scalps were thoroughly cleaned with chlorhexidine three times to avoid bacterial infection

Perfusion was performed by flushing pre-warmed RPMI containing phenol red and L-glutamine through a 25- and 3/8-gauge needle placed into the aorta attached to Tygon tubing aided by the Masterflex L/S perfusion pump as described 81

The results provide insights into the benefits and risks of GLP-1RAs and may be useful for informing clinical care and guiding research agendas

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