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The follicles were cultured for 9 days with varying CT concentrations (0.5 to 50 M)

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To determine whether the gut microbiota could reverse the side effects such as that caused by TZP on bone mineral density, blood sugar reduction and weight loss, we collected fecal samples from female db/db mice and age-matched control ones treated with TZP for 6 weeks and performed high-throughput gene sequencing for 16S ribosomal RNA (rRNA) using fecal bacterial DNA