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Webster, D

Marrocco et al

Over exposure to oxidative stress, however, can result in the imbalance of the GSH level and lead to cancer, aging, cardiovascular disease, neurodegenerative diseases and other ailments 6,7,8

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Mller cells are activated by GSH We have developed different types of retinal cultures to study neuronal-glial properties: (a) Mixed neuronglial cells (prepared in high density, with ~20 10 6 cells), (b) enriched neuronal cells prepared in low density, with ~2 10 6 cells or less, seeded on treated Poly-L-lysine (10 g/mL) plastic dishes and (c) purified Mller glia culture (510 6 cells) in DMEM containing 10% FCS and cultured for 10 days, when neurons are eliminated [17]