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NH 4 + released, -ketoglutarate formed NAD Glutamate Dehydrogenase + or NADP + required reversible one of only three reactions in humans that can fix NH by the reverse reductive amination of -ketoglutarate 4 + , i.e., covalently link it to carbon in mitochondria of most cells Some amino acids for which transaminases exist release their nitrogen more frequently as NH 4 + Deamination by dehydration serine (enzyme = serine dehydratase