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Another dCas9 fusion is the synergistic activation mediator (SAM) system, which consists of three total components: dCas9 fused to VP64 with a nuclear localization sequence (NLS) (NLS-dCas9-VP64), two activation domains (p65 and human heat-shock factor 1 (HSF1)) fused to the MS2 bacteriophage coat protein (MS2-p65-HSF1), and the sgRNA fused to two MS2 protein-targeting hairpin aptamers at the stem-loop 2 and tetraloop regions (sgRNA2.0) [70]

Denissova NG, Nasello CM, Yeung PL, Tischfield JA, Brenneman MA

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Van Langendonckt A, Casanas-Roux F, Donnez J

Menopause is associated with low ovarian but increased peripheral estrogens and a relative rise in androgen levels