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At the top of the figure, folate is reduced to dihydrofolate and then further reduced to tetrahydrofolate, which enters a circular pathway

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In our hands, consumption of NADPH was independent of the presence of strep-MBP-ROXY9 or strep-MBP-ROXY9-CPYC

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This finding accounts for the important observation that the c-met binding affinity of LASSS/peroxynitrite-treated HGF (at the 1:8000:2000 molar ratio to HGF, with the wash-out of un-reacted free LASSS) was significantly higher than the original level of un-treated positive control HGF (Figure 2d, bars 2 and 6 )