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Supernatants were purified by affinity chromatography using a Protein A MabSelectSure column (GE Healthcare) and compounds were analysed for aggregation and degradation by SDS-PAGE and SEC-HPLC using a TSKgel G3000SWxl column (Tosoh Bioscience)

The researchers concluded that IGF-I promotes an average increase of 15% in muscle mass and a 14% increase in strength in muscle tissue

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While these medications are revolutionary for weight management and metabolic health, they can present a paradoxical relationship with sleep: recent research shows that insomnia ranks as the most common psychiatric adverse event reported with GLP-1 medications, affecting users through multiple complex mechanisms