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These may include: Fat freezing for targeted reduction of localised fat deposits Laser-based fat reduction treatments Muscle-toning and body-shaping technologies such as EmSculpt Neo Biostimulation technologies such as Redustim, which support muscle activation and metabolic health These treatments are designed to enhance and refine results, rather than replace medical weight management

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The Lipofectamine 3000 Kit (L3000015, Thermo Fisher Scientific) was used to transfect the cells with two plasmids: the 2.5 g negative control plasmid pcDNA3.1 and the 2.5 g pcDNA3.1 MPO plasmid

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found that willingness to pay for weight loss drugs varies widely based on income