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When AGK is associated with PTEN the phosphatase activity of PTEN is inhibited which allows the PI3K, AKT/PKB, and mTORC1 signal transduction pathways to remain active

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In brief, serial dilutions of G49 and Fc-GLP1 in assay buffer (as above) were added in duplicate to CHO cells recombinantly expressing human GLP-1R and incubated for 30 min at RT before detection with the cAMP dynamic 2 HTRF assay