The 3D spheroids were cultured in ultralow attachment flasks (Corning, NY) in sphere medium composed by serumfree DMEM/F12 supplemented with fibroblast growth factor-basic (b-FGF, 20 ng/ml), epidermal growth factor (EGF, 20 ng/ml), insulin (20 g/ml), d-glucose (0,5%), heparin (5 g/ml), l-glutamine (2,5 mM), Penicillin-Streptomycin (0,1%) (Sigma), and B27 (0,1%) (Gibco, Invitrogen, Carlsbad, CA)
The unique challenge of nasal spray testing is that the delivery device the pump, actuator, bottle, and valve is inseparable from the drug products performance
Farah A et al
1988;27 Suppl 1:86-93
Use our versatile skin brightening product as your daily body lotion in the comfort of your home