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Notably, ILC2 proportions were comparable between IL-33 alone and IL-33 plus melatonin and Luzindole groups ( Figures 2K, L ), yet BALF concentrations of IL-5 ( Figures 2M ) and IL-13 ( Figures 2N ) remained significantly reduced with melatonin treatment, again indicating dampened ILC2 activity rather than altered ILC2 abundance

Therefore, while existing evidence supports low local toxicity, comprehensive in vitro and in vivo immunogenicity testing remains advisable prior to clinical translation [71, 191]

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