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FOXO4-DRI alleviates testosterone secretion insufficiency in naturally aged mice Our finding that FOXO4-DRI targeted senescent TM3 Leydig cells in vitro prompted us to investigate its therapeutic effect in vivo using naturally aged (20-24 months) male mice as subjects

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The tGSH concentration was quantified using the enzymatic recycling method in which rates of 5-thio-2-nitrobenzoic acid (TNB) formation were calculated after the addition of the DTNB (1.8 mM) solution (Ellmans reagent) and glutathione reductase (250 units per ml) solution to the samples

10.1016/s0021-9258(18)92922-4 25 KritzingerE

Zein et al., 2024)