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Proc Natl Acad Sci USA 103(52):1990819912

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Due to the general mechanisms such as plasmid exchange and selection pressure, bacteria resist the commonly used antibiotics, elevating the need to develop new antibiotics and modes of using them

Strains were pre-cultivated on YPD liquid medium for 24 h, and cells were then transferred into each compartment at an initial OD 610 of 0.01