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NADH-fuelled CI respiration was also rhythmic in synchronized adipocytes (Extended Data Fig

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To test whether binding of one IGF1 molecule to the IGF1R dimer is sufficient for receptor activation, we transfected HEK293 cells with the IGF1R WT plasmid alone or with a 1:1 mixture of the IGF1R WT plasmid and a plasmid that encodes IGF1R F701A, a mutant deficient in IGF1 binding