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The dried samples were reconstituted using 200 HPLC mobile phase (10% v/v ACN in water), vortexed briefly, and subjected to HPLC separation and analysis

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10.1038/bjc.2017.193 38 LirussiF.AzzaliniL.OrandoS.OrlandoR.AngelicoF

Two-colour nanometre-scale resolution imaging by Minimal Photon Fluxes (MINFLUX), an ultra-high resolution microscopy technique that combines aspects of single-molecule localisation microscopy and stimulated emission depletion (STED) microscopy 14 , was employed to determine the average distance between endosomal SNAP/FLAG-hGLP-1R and VAPB, estimated at around 28 nm (Fig